Coding
Part:BBa_K2286011:Design
Designed by: Licong He Group: iGEM17_UESTC-China (2017-10-20)
HheC-F12Q-F186L-W249P: halohydrin dehalogenase site-directed mutant
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 718
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence is codon-optimized for E.coli.
Source
This gene is from Agrobacterium radiobacterAD1.